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Enzymatic Synthesis of 2-Phenoxyethanol Galactoside by Whole Cells of ${eta}$-Galactosidase-Containing Escherichia coli
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  • Enzymatic Synthesis of 2-Phenoxyethanol Galactoside by Whole Cells of ${eta}$-Galactosidase-Containing Escherichia coli
  • Enzymatic Synthesis of 2-Phenoxyethanol Galactoside by Whole Cells of ${eta}$-Galactosidase-Containing Escherichia coli
저자명
Lee. Hyang-Yeol,Jung. Kyung-Hwan
간행물명
Journal of microbiology and biotechnology
권/호정보
2014년|24권 9호|pp.1254-1259 (6 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

We investigated whether ${eta}$-galactosidase (${eta}$-gal)-containing Escherichia coli cells could transfer a galactose to 2-phenoxyethanol, resulting in 2-phenoxyethanol galactoside (PE-Gal). PE-Gal was confirmed by liquid chromatography-mass spectrometry. In addition, we also confirmed that a galactose molecule was covalently bonded with PE during thin-layer chromatography analysis of the ${eta}$-gal hydrolysate of PE-Gal. The yield for PE-Gal synthesis was about 37.5% (weight basis), which was about 7-8 times greater than that of a previous report. In addition, the concentration of ${eta}$-gal (0.96 U/ml) used in this PE-Gal synthesis was about 20 times less than that in a previous report.