Objectives: The pulse diagnosis to identify the symptoms has been considered important in Korean medicine. The position and character of disease would be confirmed by pulse diagnosis of left and right radial artery. This paper is to analyze the characteristics and differences of left and right blood vessels. Methods: In this study, left and right radial artery and dorsalis pedis artery was measured and analyzed by using condenser typed pulse analyzer. Commercially available pulse analyzer was used to measure the radial artery. The pulse wave was measured in 20 laboratory healthy men and women. The blood vessel aging degree and index of augmentation of blood vessel was obtained from the measured pulse wave graph and the characteristics and differences of the left and right blood vessel was analyzed. Results: The significant difference of pulse transit time between the right handed and non-right handed was not found. The correlation of radial artery and dorsalis pedis artery had no significant difference. By obtaining the blood vessel aging index (AGI) and augmentation index (AI) of blood vessel at the left and right radial artery, the significant difference between right handed and non-right handed was not found. Conclusions: The result of this study would help to explain the characteristic of blood vessel with respect to the left and right handed. We suggest that research of pulse wave of the left and right blood vessel using pulse analyzer should be needed in further study.