Purpose: This study set out to develop a machine for separating shatter-resistant sesame after threshing. Methods: Three grades of sieve and different blower speeds were tested for a separation system that had been designed specifically for shatter-resistant sesame. Performance tests were run to evaluate the sieve and blower systems in terms of the sesame separation and loss ratios. Results: Tests of the first separation stage using the sieve system revealed the optimum sieve perforation size to be 5 mm. Tests of the second separation stage using the blower system identified the optimum blower speed as being 220 rpm. The optimum separation and loss ratios, of 96.5% and 3.5%, respectively, were obtained at a blower speed of 220 rpm. Conclusions: These results will be useful for the design, construction, and operation of threshing harvesters. For shatter-resistant sesame, an optimum blower speed of 220 rpm was identified.