Blood transfusion is generally the process of receiving blood products into one’s circulation intravenously. Transfusions are used for various medical conditions to replace lost components of the blood. Early transfusions used whole blood, but modern medical practice commonly uses only components of the blood, such as red blood cells, white blood cells, plasma, clotting factors, and platelets. Cross-matching blood, in transfusion medicine, refers to the testing that is performed prior to a blood transfusion in order to determine if the donor’s blood is compatible with the blood of an intended recipient, or to identify matches for organ transplants. Major cross-match compares donor erythrocytes to recipient serum in EDTA or citrate and serum from the recipient Minor cross-match compares donor serum to recipient erythrocytes and checks for preformed antibodies in donor serum that could hemolyse recipient red cells. This cross-match is less important as usually the donor serum is markedly diluted after transfusion and is unlikely to produce a significant transfusion reaction. This study was conducted from september to october 2013 in Jeju Island. A total of 56 tests were couducted to target segmanet concentration of red blood cells. Randomly sampled segments the Direct Coombs test. Packed red cells in the 56 segmented Ortho BioVue system (Ortho-Clinical Diagnostics, USA). The result is positive for the four samples (7.14%), respectively. 1 positive grade for the two specimens showed that the two samples showed a weakly positive grade. In this study, the Direct Coombs test is positive donors if the transfusion because it affects cross-matching test cross examination of a blood transfusion, if it seems inadequate to feed that should not be overlooked.