The total global emission of CO2 from soils is recognized as one of the largest fluxes in the global carbon cycle. Especially it is necessary to quantify the amount of CO2 emitted by the organic material decomposition processes of microorganisms in the soil, because it becomes one of a factor for determining the carbon stocks in the soil. This study was conducted to estimate the impact of the Korean water deer(Hydropotes inermis)’ feces to the soil organic matter. Also, effects of Korean water deer’ feces on CO2 emissions of soil and land use pattern dependent CO2 flux quantification are studied. The organic materials in the Korean water deer’ feces significantly changed organic matter content of soil and influenced the activity of soil microorganisms, both changing of respiration of the soil and physical·chemical components in soil. In particular, C/N ratio and the CO2 flux of soil of four regions (Rice paddy, Fallow ground, Salix koreensis community, Phragmites australis community) showed a statistically highly significant correlation (P<0.01) with the presence or absence of feces. CO2 flux of soil affected by the feces was 2-20 times higher than the soil unaffected by the feces. This study has great significance to quantify the extent of the material circulation and its impact to the terrestrial ecosystem and soil zone throughout Korean water deer’ feces. Feces of wildlife can affect soil and soil material circulation.