The effects of song activities on the changes of brain concentration ratio and the brain activation were investigated using neurophysiological electroencephalography (EEG) index. A total of 78 subjects (37 male, 41 female, mean age 27.00±8.35 years) were randomly assigned to two groups by the type of stimulus. In order to investigate the changes between singing and song listening, changes between singing and oral reading, EEGs of all subjects were recorded during resting, and were also recorded immediately after performing three different active tasks, including singing, song listening, and oral reading tasks. The performing orders were randomly chosen for active tasks. EEGs in four conditions, including during resting, after singing, after song listening, and after oral reading, were measured using EEG measurement system (PolyG-I) with electrodes placed in eight channels of Pre-frontal Lobe (Fp1, Fp2), Frontal Lobe (F3, F4), Temporal Lobe (T3, T4), and Parietal Lobe (P3, P4). The changes of brain concentration ratio (Ratio of SMR-Mid Beta to Theta, RSMT) and total changes of brain activation (Absolute Total Power, ATP) were statistically analyzed at the 5% significance level with paired t-test from the collected EEG signals. The results indicated that the level of both RSMT and ATP in singing were increased more than those in other conditions including song listening and oral reading tasks in both groups. These findings suggested that familiar singing activity resulted in positive responses on improving the level of both RSMT and ATP.