This study was conducted to investigate their relationship between job stress of daycare center teachers and organizational trust, and to figure out whether job engagement and organizational commitment influence as regulatory factors among the relationships, and to provide scientific data in terms of effectively managing child-care teachers. As a result of the analysis, the overall level of job stress of nursing teachers was not significantly high, and the level of trust on co-teachers was the highest. There was no significant difference between the levels of job engagement and organizational commitment. It was confirmed that every sub-factors of nursing teacher’s job stress exercised negative(-) influence organizational trust, and infants and children protection and job stress were the greatest influencing factors. In particular, the results indicated that job stress exercised the most negative influence on trust in organization of daycare centers and principals. In addition, the hypothesis of job stress and organizational commitment being moderating factors in the relationship between job stress and organizational stress is confirmed.