The flood pulse in streams enhances the biodiversity and ecosystem services of the channel-floodplain ecosystems by exchanging water, nutrients, sediments and organisms. However, the lateral connectivity in most streams of Korea has been disrupted by the levee constructions for the purpose of flood control and land use of floodplains. To compare the characteristics of floodplain vegetation according to existence of lateral connectivity in streams, we investigated the geomorphological and soil environmental factors and structures and distribution of vegetation in the floodplains connected and isolated by levee to the channel in Cheongmi Stream, Seom River, Hwangguji Stream, Mangyeong River, Gomakwon Stream, and Boseong River, Korea. In comparison of soil environments, moisture and clay contents were higher in the isolated floodplain than in the connected floodplain. According to the result of principal component analysis (PCA) using environmental data, the environments of the connected floodplain and the isolated floodplain were separated by soil moisture contents, soil texture and distribution altitude of the vegetation. The results of detrended correspondence analysis (DCA) using vegetation data showed that the isolated floodplain was dominated by the hydropythic communities of diverse life form and that the connected floodplain was dominated by the hygrophytic communities that endure disturbance. In conclusion, it is thought that the vegetation of the floodplain changed to the lentic wetland vegetation dominated by diverse hydrophytes as the floodplain was isolated from the channel by artificial levees.