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Push-in Head Restraining Apparatus for Intracranial Self Stimulation Tasks in Rats
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  • Push-in Head Restraining Apparatus for Intracranial Self Stimulation Tasks in Rats
저자명
MootaekRoh,Il-SungJang,Maan-GeeLee
간행물명
The Korean Journal of Physiology & PharmacologyKCI
권/호정보
2014년|18권 2호(통권104호)|pp.103-108 (6 pages)
발행정보
대한생리학회-대한약리학회|한국
파일정보
정기간행물|ENG|
PDF텍스트(1.38MB)
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영문초록

Head restraining is an experimental technique that firmly secures the animal’s head to a fixation apparatus for the precise control and sensing of behaviors. However, procedural and surgical difficulties and limitations have been obstructing the use of the technique in neurophysiological and behavioral experiments. Here, we propose a novel design of the head-restraining apparatus which is easy to develop and convenient for practical use. Head restraining procedure can be completed by sliding the head mounter, which is molded by dental cement during implantation surgery, into the port, which serves as matching guide rails for the mounter, of the fixation bar. So neither skull-attached plates nor screws for fixation are needed. We performed intracranial self stimulation experiment in rats using the newly designed device. Rats were habituated to acclimatize the head-restraint environment and trained to discriminate two spatially distinguished cues using a customized push-pull lever as an operandum. Direct electrical stimulation into the medial forebrain bundle served as reward. We confirmed that head restraining was stable throughout experiments and rats were able to learn to manipulate the lever after successful habituation. Our experimental framework might help precise control or sensing of behavior under head fixed rats using direct electrical brain stimulation as a reward.

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