This study was to examine changes in relational responds of self report on the Student-Teacher Relationship Scale and three subscales: conflict, closeness, dependency. In order to assess professional qualifications, the differences between before and after formal training was analyzed. Participants were 135 teachers of public and private kindergarten in Korea. The results were first, the sensitivity of professional quality on the scale of Korean kindergarten teachers was reported higher conflict subscale, and lower closeness and dependency subscale. Second, there was no differences of professional qualifications between before and after formal training. As the quality of professionals administrating, scoring and interpreting the Scale should have formal training in standardized testing.