Purpose: This study was conducted to identify the influence of nursing satisfaction in major, and gender role conflicts on career preparation behaviors among male nursing students. Methods: The subjects were 527 male students in nursing college. Data were collected from self-reported questionnaires and analyzed by descriptive statistics, Pearson corrleation coefficient, and stepwise multiple regression. Results: The average score of satisfaction in major, gender role conflicts, and career preparation behavior was 3.91, 3.04, and 2.40 respectively. There were positive correlations among satisfaction in major, gender role conflicts, and career preparation behavior. These variables together explained 16% of the career preparation behavior in male nursing students. Conclusion: In order to connect the satisfaction in major with career preparation behaviors, the school and the department will need a lot of efforts and support for developing educational curriculum that can meet the students level and interst, and practical training for clinical practice.