The purpose of this study is to develop a Brain Education based Kigong program to reduce stress and depression in middle – aged women and to verify the effectiveness of the program. We used the questionnaire scale to measure the degree of stress and the questionnaire scale to measure the degree of depression as a tool to measure the dependent variables and analyze the blood to confirm their biochemical changes. The subjects of this study were 40 female (40 experimental group, 20 control group) who were in the 40 ~ 60 age group of nutrition team at oo university in Jeonnam. Both the experimental group and the control group were given pre – and post - stress questionnaire and depression scale questionnaire and blood was collected. The experimental group performed the Brain Education based Kigong program for 12 weeks, twice a week (50 minutes), and the control group did not. Data were analyzed using independent sample t-test, paired t-test, and two-way ANOVA analysis. The results of the study showed that Brain Education based Kigong programs reduced the degree of depression in middle – aged women and did not show a significant decrease in stress level. Blood analysis showed that insulin, total cholesterol, and LDL cholesterol showed a higher rate of decrease in the experimental group compared to the control group, but no statistically significant difference was found. These results suggest that Brain Education based Kigong program may have a effectiveness on depression in middle – aged women, but the identification of biological indicators should be clarified in future studies.