Purpose: The purpose of this study is to investigate the awareness of biomedical ethics of nursing college students and non-nursing college students and to examine their attitudes toward euthanasia. Methods: The study design was a descriptive research study and analyzed 247 university students attending a university in G city. Data were analyzed using SPSS 23.0 program. The general characteristics of the major were determined by number, percentage, and χ²-test. The difference between the awareness of biomedical ethics and attitude of euthanasia was analyzed by t-test. Results: As a result of this study, there was no statistically significant difference in the awareness of biomedical ethics according to the subjects of the study(t=1.55, p=.124), but Statistical significance was found only in the sub-domain artificial abortion(t=2.33, p=.021). In addition, there was no statistically significant difference in attitude toward euthanasia according to subjects major(t=-1.37, p=.171). Conclusions: In order to establish a desirable ethical structure as a society member of the future, university students should provide systematic education and various programs on euthanasia in addition to the biomedical ethics awareness.