Objective: The purpose of the present study was to examine the moderating role of childcare teachers’ sense of humor in the relationship between their job stress and happiness Methods: Participants were 294 childcare teachers working at 83 childcare centers located in Busan and Gyeongnam. Teachers completed a rating scale in order to examine their demographic variables, job stress, sense of humor, and happiness. The collected data were analyzed using product-moment correlation and hierarchical multiple regression. Results: Childcare teachers’ job stress was negatively correlated with their happiness. And childcare teachers’ sense of humor had a positive relationship with their happiness. Hierarchical regression analysis indicated that the interaction of childcare teachers’ job stress and sense of humor predicted their happiness. Childcare teachers’ job stress was significantly associated with their happiness among those who demonstrated the lowest level of sense of humor. Childcare teachers’ job stress had no relationship with their happiness, especially for those with the highest level of sense of humor Conclusion/Implications: We suggested that having a sense of humor might be valuable for childcare teachers in order to buffer the link between their job stress and happiness.