Objective: The purpose of the present study was to examine the mediating role of career calling in the relationship between job stress and burnout as well as the association among job stress, career calling, and burnout. Methods: A sample of three-hundred and seven child care teachers in the metropolitan area of Seoul completed questionnaires on job stress, career calling, and burnout. Results: Correlation and regression analyses indicated that lack of directors’ leadership and administrative support, work overload, and career calling significantly predicted child care teachers’ burnout. Moreover, the association of lack of directors’ leadership and administrative support of burnout was mediated by career calling. Conclusion/Implications: The findings suggest that directors’ leadership and seamless administrative support need to be emphasized in order to reduce burnout and mitigate work-related stress among child care teachers. The findings also highlight the important role of career calling in reducing burnout among child care teachers and suggest the consideration of career calling as a target in interventions and educational programs.