Fetal inflammatory response syndrome is a multisystemic disorder, defined by elevated interleukin-6 (IL-6) in fetal plasma and associated with preterm delivery and adverse neonatal outcomes. Recent evidences indicate that the elevated concentration of matrix metalloproteinase-8 (MMP-8) is associated with fetal inflammatory response syndrome. Nuclear factor kappa B (NF-κB), has central roles in human immune response. In this study, we made in vitro model of fetal inflammatory response syndrome using lipopolysaccharide (LPS) stimulation to cord blood. The purpose of this study was to demonstrate that LPS induces a release of IL-6 and MMP-8 as well as NF-κB in cord blood. LPS stimulation increased the expression of NF-κB in a time dependent manner. MMP-8 level was significantly correlated with IL-6 level. All these results directly support that IL-6 and MMP-8 could be a useful marker for the diagnosis of fetal inflammatory response syndrome.