페룰산 함량이 붉은곰팡이병과 곰팡이독소 저항성 곡류 육종의 선발지표가 될 수 있는지 알기 위해 밀, 보리, 벼 육성 품Table 4. Comparison of barley and wheat for trichothecenes produced by head blight pathogens Pathogena Trichothecene level (μg/g)b t value P-value Wheat Barley Fg(PH-1)-DON 3.86±2.92 6.16±0.28 -2.12051 0.078 Fg(Z34)-DON 3.48±2.62 4.77±2.07 -0.85405 0.426 Fa(W1D)-DON 4.41±0.77 4.75±0.73 -0.74320 0.485 Fa(#73)-NIV 3.04±2.54 5.90±0.60 -2.97238 0.025 Mean 3.69±1.51 5.40±0.40 -2.64047 0.039 aSpecies (isolate name)-trichothecene chemotype: Fg, Fusarium graminearum; Fa, F. asiaticum; DON, deoxynivalenol; NIV, nivalenol. bCalculated as a mean of trichothecene levels±standard deviation. Fig. 2. Trichothecenes produced by head blight pathogens in barley and wheat cultivars and ferulic acid content. The cultivars with w and b indicate wheat and barley, respectively. The pathogen names appear in parentheses. DON, deoxynivalenol; NIV, nivalenol; Mean, mean of toxin levels; FA, ferulic acid. Research in Plant Disease Vol. 26 No. 4 255 종 80점의 페룰산 함량을 조사하였다. 페룰산 함량은 보리 20품종이 1.66‒2.77 mg/g, 밀 40품종이 0.56‒1.53 mg/g이었으며 벼20품종은 0.91‒2.13 mg/g이었다. 이 중 페룰산 함량이 다른 밀과보리 각 7품종을 선정하여 F. graminearum과 F. asiaticum 각 2균주의 붉은곰팡이병과 독소 생성량을 분석하였다. 밀 품종의 평균 병원성은 페룰산 함량이 높은 보리에 비해 유의하게 낮았고밀 품종 간에는 상관관계가 없었다. 곰팡이독소도 병원성과 같이 페룰산 함량이 낮은 밀이 보리보다 생성량이 낮았다. 그러나보리에서는 F. asiaticum 균주의 병원성과 독소 생성량이 페룰산 함량과 음의 상관관계를 나타내었다. 이 결과는 페룰산 함량이 밀과 보리에서 F. graminearum과 F. asiaticum에 의한 붉은곰팡이병과 독소 생성에 저항성 요인이 아님을 보여준다.
In order to find if a ferulic acid (FA) can be used as a selection index in cereal breeding for resistance to head blight and mycotoxin production, we analyzed FA in the grains of 80 cultivars of barley, rice, and wheat. FA content ranged 1.66-2.77 mg/g in barley (n=20), 0.56-1.53 mg/g in wheat (n=40), and 0.91-2.13 mg/g in rice (n=20). Among these, 7 cultivars each of barley and wheat with different FA content were tested for head blight and mycotoxin production by 2 Fusarium graminearum and 2 F. asiaticum strains. Mean pathogenicity of the wheat cultivars was significantly less than that of barley with higher FA and among wheat cultivars, there was no correlation between FA content and pathogenicity. Mycotoxin production was also lower in the wheat than in the barley as pathogenicity. However, pathogenicity and toxins produced by F. asiaticum were negatively correlated with FA content in barley. These results indicate that FA is not a resistance factor to head blight by F. asiaticum and F. graminearum or its mycotoxin production in barley and wheat.
재료 및 방법 결과 및 고찰 요 약
