Purpose: The goal of this study was to see how different aerobic exercise intensities affected AMP-activated protein kinase (AMPK), reactive oxygen, and antioxidant enzymes in young mice during an 8-week period. Methods: Forty male C57BL/6 mice, aged seven weeks, were randomly assigned to one of four groups: control (n= 10), low-intensity exercise (n= 10), moderate-intensity exercise (n= 10), and high-intensity exercise (n= 10). For eight weeks, aerobic activity was performed once a day for 35–40 minutes, five days a week. The data were analyzed using descriptive statistics, analysis of variance (ANOVA), chi-squared tests, and the Tukey test in the SPSS/WIN 25.0 program. Results: Weight (p= .001) was substantially different between the moderate-intensity exercise group and the control group in AMPK (p< .001). In addition, there were no significant differences between the moderate-intensity exercise group and the control group in reactive oxygen malondialdehyde (MDA) levels (p= .136) and antioxidant enzyme superoxide dismutase (SOD) levels (p= .521). Conclusion: These findings suggest that moderate-intensity aerobic exercise increased AMPK activation and helped young mice shed weight.