This study examined the effect of touch play art therapy on the attachment and self-regulation of middle entry infants. Five years middle-entry infants participated in a total of 20 art therapy sessions once or twice a week for 60 minutes in each session from January to March 2021. To assess the effect of their attachment and self-regulation, researchers conducted the Child Attachment interview, Dynamic Family Drawing, Bird’s Nest Drawing, and Self-Regulation Scale and observed their changes through art therapy activities. First, the child attachment test results showed positive changes in attachment feelings and behavior treatment. Dynamic familiarization enhanced family bonding, interaction, and parental support, while bird nesting result showed a shift from low to stable attachment levels. Second, self-regulation ability test result showed that self-control was improved, impulsivity was decreased, and attention was enhanced, which had a positive effect on infant self-regulation ability. Touch play art therapy fostered positive changes in attachment and self-regulation. Initially. These infants expressed their anxiety, tantrums, aggression, and impulsivity, whereas their self-regulation was enhanced during art therapy. This study intended to provide basic data for understanding the impact of attachment formed through touch play art therapy for self-regulation ability. Based on these research findings, the implications and limitations of this study were discussed.