Colletotrichum musae was isolated from dark-brown anthracnose lesions on commercial banana (Musa sapientum L.) to establish the causal agent of the symptom. The fungus grew fast and produced white aerial mycelium on PDA. Acervuli developed abundantly on culture plates after incubation for 10 days at 25℃. Pinkish conidial masses were produced on the acervuli, which mostly coalesced together. Conidia were aseptate, hyaline, straight, ellipsoid to globose, and 14.5 × 6.9 mm in size. Black, clavate, round, or irregular-shaped appressoria measuring 8.8 × 6.8 mm were readily formed from germ tubes. Setae-like structures were not found either on the lesion or on the cultures. Sclerotia were also absent. Among the media, PDA medium was the best for mycelial growth. The optimum temperature for mycelial growth was 28℃, while the optimum pH ranged from pH 5.5 to 6.5. The isolates of C. musae caused black necrotic lesions on banana fruits by needle-wound inoculation, and orange-colored spore masses were produced on the lesions. The fungus also caused discoloration on apple fruits inoculated.