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dsRNA Analysis and Sequence of S12 to Rice dwarf virus Korean Isolate
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  • dsRNA Analysis and Sequence of S12 to Rice dwarf virus Korean Isolate
저자명
Bong Choon Lee, Do Yeon Kwak, Yeon Kyu Hong, Hyun Je Cho, Sung Tae Park, Soon Chul Kim
간행물명
The Plant Pathology Journal KCI
권/호정보
2004년|20권 2호(통권90호)|pp.155-157 (3 pages)
발행정보
한국식물병리학회|한국
파일정보
정기간행물|ENG|
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영문초록

We isolated Rice dwarf virus (RDV) from infected plants in rice fields (Korea, Japan, China, the Philippines and Nepal) and analyzed their genomic dsRNAs by polyacrylamide gel eletrophoresis. The genomic dsRNAs of the isolates showed distinct electrophoretic mobility profiles. The S12 coding to nonstructural protein of Korean isolate (RDV-Kr) was further analyzed by sequencing. The S12 of RDV-Kr was 1,066bp long and coded for a protein composed of 312 amino acids including three open reading frames of P12, P12OPa and P12OPb. The sequence identities were 96% and 98.6% with Japanese isolates (H, AN), 94.7% with Nepalese isolate (NEL), 94% with Chinese isolate (CK) and the Philippines isolate (P).

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