Purpose: Dyslipidemia is one of the most important cardiovascular risk factors. The purpose of this study was to compare serum lipid levels in college students and pre-graduates. Methods: Data were obtained from student health examination in one university located in Kyunggi-province. Participants were 440 college students and 1973 pre-graduates. The data were collected in May of 2010. T-test, Chi-square test, Fisher’s exact test, and multiple regression with dummy variables using SAS version 9.1 were performed. Results: Pre-graduates showed significantly higher level of total cholesterol, triglyceride, and LDL than college students. There was no significant difference in alcohol drinking between college students and pre-graduates. However, smoking and diet behavior were significantly different; pre-graduates were more likely being current smoker and recommended to change diet behavior. When adjusting covariates including general and behavioral characteristic, pre-graduates had significantly higher total cholesterol (p<.001), triglyceride (p=.003), and LDL (p=.003). Among covariates, smoking was significantly related to triglyceride, particularly past smokers. Conclusion: There is a need to develop a lipid-reducing program targeting pre-graduates. This program should be considered behavioral factors, particularly smoking. In addition, given the fact that pre-graduates had unhealthy habits compared with college students, interventions for correcting health behaviors in this population are required.