Purpose: This study was conducted to test the effect of the 12 weeks brisk walking exercise program on body composition, blood pressure, blood glucose and blood lipid for middle-aged woman with obesity. Method: The subject were 25 obese women in 40-64 yr old who were composed of over 30% body fat. For the analysis, descriptive statistics, paired t-test were used for statical analysis with SPSS 15.0. Result: The results were summarized as follows: first, the body weight, body mass index (BMI), % body fat of the obese woman were significantly decreased after implementing the 12 weeks brisk walking exercise program (p＜.5). Second, the systolic blood pressure, diastolic blood pressure and blood glucose were significantly decreased after the 12 weeks brisk walking exercise program (p＜.5). Third, the serum cholesterol was significantly decreased (p＜.05) but serum triglyceride was not significantly decreased the after 12 weeks brisk walking exercise program. Conclusion: These results suggest that the brisk walking exercise program has an effect on decreasing body weight, BMI, percent body fat, blood pressure and serum cholesterol in middle-aged woman with obesity to reduce obesity and prevent chronic disease.