This study was conducted with the primary-school student subjects whose brains still underwent development, with the purpose of identifying effect of the school physical class duration of exercises on the expression of BDNF, IGF-1 & Healthrelated fitness. The subjects of this study are 30 students with no history of physical illness from primary school which is located in Gyeonggi do Republic of Korea. The vaulting horse events were selected physical education classes. All students to run the same number of activities were controlled. Physical education class were performed 3 times per week in the duration of 8 weeks using vaulting horse and class time was set at 40 minutes. Physical class after of exercises the change in serum BDNF and IGF-1 showed a statistically significant difference(p<.05), (p<.05). There were showed a statistically significant difference in Cardio-respiratory endurance, muscular strength, agility, body fat(%)(p<.05)(p<.05(p<.05)(p<.05). Thus, it is believed that school physical class of exercises will have positive effect on the enhancement of Health- related fitness and the serum BDNF levelst and IGF-1 of the primary school students who are still going through brain developments.