항생제 내성균 및 유전자제거를 위한 염소 CT 값 비교

오준식; 김성표

서지반출

국문초록

본 연구의 목적은 항생제 내성 균과 유전자 및, 항생제 내성 전달을 제어 하는데 필요한 살균능 (CT, 농도 * 접촉 시간)을 서로 비교하는데 있다. 이를 위하여, 이를 위해 각기 다른 염소 주입농도(C)와 접촉시간(T)에 따라 각각의 항생제 내성 제거를 산정하였다. 그 결과 항생제 내성균 90%(1 log)이상 제어를 위해서는 CT 값( 176∼353 mg·min/L)이 필요하였으며, 항생제 내성 유전자의 제거를 위한 CT 값은 195∼372 mg·min/L 이 었다. 또한 항생제 내성 유전자의 전이 90% 이상 제거를 위한 CT 값은 187∼489 mg·min/L이었다. 따라서, 본 연구조건에서는 항생제 내성 유전자 및 유전자의 전이에 대한 제어를 위해서는 항생제 내성균 제어보다 더 높은 소독능이 필요함을 알 수 있었다.

영문초록

The purpose of this study is to compare CT (disinfectant concentration * time) values in removing the antibiotic resistance bacteria, antibiotic resistance gene and transfer of antibiotic resistance genes. Different concentration of chlorine(C) and contact time(T) according to the removal of antibiotic resistance was calculated for each. As a result, for the 90% removal of antibiotic resistant bacteria, around 176∼353 mg·min/L CT values are needed. For the removal of the antibiotic resistance gene, 195∼372 mg·min/L CT values are required. For the 90% reduction of antibiotic resistance gene transfer by chlorine disinfection, 187 ∼489 mg·min/L CT values are needed. Based on our results, higher CT value was required for removing antibiotic resistant genes rather than antibiotic resistance bacteria.

키워드

CT 값 염소소독 항생제 내성균 Chlorine disinfection Escherichia coli DH 5 alpha(E.coli/DH5α)pB10

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