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전체 선택해제
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Activation of K+ channel by 1-EBIO rescues the head and neck squamous cell carcinoma cells from Ca2+ ionophore-induced cell death
MingZheYin, Seok-WooPark, , TaeWookKang, KyungSooKim, , , HaeYoungYoo6, JunhoLee, J.HunHah, , MyungHunSung, , SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.1 4 25-33 (9 pages)
Ion channels in carcinoma and their roles in cell proliferation are drawing attention. Intracellular Ca2+ ([Ca2+]i)-dependent signaling affects the fate of cancer cells. Here we investigate the role of Ca2+-activated K+ channel (SK4) in head and neck squamous cell carcinoma cells (HNSCCs) of dif-ferent cell lines; SNU-1076, OSC-19 and HN5. Treatment with 1 μM ionomycin induced cell death in all the three cell lines. Whole-cell patch clamp study suggested common expressions of Ca2+-activated... -
괴화(槐花) 에탄올 추출물이 RBL-2H3 비만세포에서 Ca++ Ionophore에 의한 알레르기 반응 조절에 미치는 효과
손효, 강군, 심도완, 김태권, 강태봉, 이광호, Xiao. Sun, Jiang. Jun, Shim. Do-Wan, Kim. Tae-Kweon, Kang. Tae-Bong, Lee. Kwang-Ho 한국식품영양과학회 한국식품영양과학회지 6 Pages
한국식품영양과학회 한국식품영양과학회지 2014, Vol.43 No.3 349-354 (6 pages)
세포내 $Ca^{{+}{+}}$의 증가는 비만세포에서 수용체 활성을 거치지 않고 탈과립을 유도한다. 괴화는 천연 염색 재료로 사용되고 있으며, 또한 항염증 작용과 $Fc{varepsilon}RI$와 IgE 가교에 의한 항알레르기 효능도 보고되었다. 이번 연구에서 비만세포에서 $Ca^{{+}{+}}$ 유입에 의해 생산되는 알레르기 매개물에 대한 괴화 추출물의 조절 기능을 보고한다. 괴화 추출물은 A23187에 의해 유도되는 IL-4와 TNF-${alpha}$의 생산과 탈과립을 저해하였다. 또한 괴화 추출물은 DNFB로 유도한 알레르기 피부염의 동물 모델에서 알레르기... -
무지개송어(Oncorhynchus mykiss) 배양 간세포에서 Vitellogenin 합성에 미치는 Calcium ionophore의 영향
한국수산과학회 韓國養殖學會誌 1998, Vol.11 No.2 241-248 (8 pages)
Vitellogenin(VTG) 합성에 미치는 A23187의 영향을 무지개송어의 배양 간세포를 이용하여 실험을 행하였다. 간세포는 2일간 배양한 후, Estradiol-$17^{eta}$($E_2$, $2{ imes}10^{-6}$M) 및 A23187 ($10^{-7)$~$10^{-5}$M)을 첨가하여 7일간 배양하였다. 그리고, $E_2$에 의한 VTG 합성시의 A23187이 미치는 영향에 대해서도 조사하였다. A23187이 미치는 영향에 대해서도 조사하였다. A23187 ($10^{-7)$~$10^{-5}$M)의 첨가에 의해 간세포에서의 VTG 합성은 농도의 증가에 따라 감소하였다.$E_2$에 의해 합성된 VTG는 A 23187... -
Calcium Ionophore를 이용한 소 배반포로 부터의 배아주 유사세포의 효과적인 분리
김선욱, 류재웅, 이철상, 한용만, 박정선, 유대열, 이경광 한국발생생물학회 발생과 생식 7 Pages
한국발생생물학회 발생과 생식 1999, Vol.3 No.1 45-51 (7 pages)
분리하기 위해서는 영양외배엽 (trophectoderm, TE) 세포를 제거하는 것이 효과적이다. 따라서 본 실험은 효과적으로 TE를 제거하기 위한 calcium ionophore A23187 (CIPA) 처리조건을 확립하고, 분리해낸 ES 유사세포의 in vitro 다능성 (pluripotency)을 검증하고자 수행하였다. CIPA 농도 및 처리시간을 달리 하였을 때 50 $mu$M에서 25분간 처리한 군이 colony 형성율 (51%)및 10 passage 까지의 배양성적 (4.8%)에서 가장 좋은 결과를 나타내었다. 또한 CIPA를 처리하지 않은 군과의 비교에서도 약 5배의 높은 결과를 보임으로서 본... -
Influence of Ionophore Supplementation on Growth Performance, Dietary Energetics and Carcass Characteristics in Finishing Cattle during Period of Heat Stress
Barreras. A., Castro-Perez. B.I., Lopez-Soto. M.A., Torrentera. N.G., Montano. M.F., Estrada-Angulo. A., Rios. F.G., Davila-Ramos. H., P 아세아태평양축산학회 Asian-Australasian journal of animal sciences 9 Pages
아세아태평양축산학회 Asian-Australasian journal of animal sciences 2013, Vol.26 No.11 1553-1561 (9 pages)
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The Novel SCN- Ion-selective Electrode Based on the 1-Benzyl-3-(4-nitrophenyl) thio-urea Ionophore
Lee. Kyungmi, Kang. Dong Hyeon, Choe. Ju Eun, Yun. Mira, You. Jung-Min, Go. Min Jeong, Lee. Junseong, Jeon. Seungwon 대한화학회 Bulletin of the Korean Chemical Society 6 Pages
대한화학회 Bulletin of the Korean Chemical Society 2014, Vol.35 No.11 3175-3180 (6 pages)
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Selective Fe2+ Ion Recognition Using a Fluorescent Pyridinyl-benzoimidazole-derived Ionophore
Lee. Jeong Ah, Eom. Geun Hee, Park. Hyun Min, Lee. Ju Hoon, Song. Hyesun, Hong. Chang Seop, Yoon. Sungho, Kim. Cheal 대한화학회 Bulletin of the Korean Chemical Society 4 Pages
대한화학회 Bulletin of the Korean Chemical Society 2012, Vol.33 No.11 3625-3628 (4 pages)
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Synthesis and Binding Properties of 1,3,5-Tris(2-arylthiomethyl)mesitylene: A Selective Ag (I) Ionophore
Kim. Hong-Seok, Bae. Seon-Yun, Kim. Ki-Soo, Choi. Jun-Hyeak, Choi. Heung-Jin, Shim. Jun-Ho, Cha. Geun-Sig, Nam. Hak-Hyun 대한화학회 Bulletin of the Korean Chemical Society 5 Pages
대한화학회 Bulletin of the Korean Chemical Society 2008, Vol.29 No.2 417-421 (5 pages)
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A Highly Selective and Sensitive Calcium(II)-Selective PVC Membrane Based on Dimethyl 1-(4-Nitrobenzoyl)-8-oxo-2,8-dihydro-1H-pyrazolo[5,1-a]isoindole-2,3-dicarboxylate as a Novel Ionophore
Zamani. Hassan Ali, Abedini-Torghabeh. Javad, Ganjali. Mohammad Reza 대한화학회 Bulletin of the Korean Chemical Society 6 Pages
대한화학회 Bulletin of the Korean Chemical Society 2006, Vol.27 No.6 835-840 (6 pages)
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The Potentiometric Studies on the Effects of Various Functional Groups in Disiloxane as an Anion-Selective Ionophore
Jung. Hyo-Jin, Lee. Myong-Euy, Lim. Chae-Yun, Paeng. Ki-Jung 대한화학회 Bulletin of the Korean Chemical Society 6 Pages
대한화학회 Bulletin of the Korean Chemical Society 2005, Vol.26 No.1 57-62 (6 pages)
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생쥐 난자성숙에 미치는 $Ca^{++}$ Ionophore와 $Ca^{++}$ Channel Blocker의 영향
배인하, 김현숙, 김문규, Bae. In-Ha, Kim. Hyun-Sook, Kim. Moon-Kyoo 대한생식의학회 大韓不妊學會雜誌 12 Pages
대한생식의학회 大韓不妊學會雜誌 1992, Vol.19 No.2 105-116 (12 pages)
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Ethanol, $Ca^{2+}-Ionophore$ 및 Strontium이 돼지 난자의 활성화와 체외 발달에 미치는 영향
안현정, 이지웅, 강만종, 문승주, Ahn. H.J., Lee. J.W., Kang. M.J., Moon. S.J. 한국수정란이식학회 韓國受精卵移植學會誌 6 Pages
한국수정란이식학회 韓國受精卵移植學會誌 2007, Vol.22 No.1 75-80 (6 pages)
ethanol, Ca-ionophore 및 strontium의 최적 농도 및 노출 시간을 규명하기 위하여 실시되었다. Ethanol은 10%에서 10분간 노출시켰을 때 난할율과 배발달 성적이 각각 51.4%와 45%로 다른 처리구에 비하여 유의적으로 높았으며 (P<0.05), Ca-ionophore는 $25{mu}M$에서 2분간 노출시켰을 때 난할율과 배발달 성적이 유의적으로 높았다. 또한 strontium은 20mM에서 6시간 노출시켰을 때 난할율과 배발달 성적 모두 유의적으로 높았다(P<0.05). Strontium중복 처리는 단독 및 병용처리 할 때보다 난할율과 배발달율 모두 유의 적으로... -
In vitro development of canine somatic cell nuclear transfer embryos in different culture media
Dong-Hoon Kim, Jin-Gu No, Mi-Kyung Choi, Dong-Hyeon Yeom, Dong-Kyo Kim, Byoung-Chul Yang, Jae Gyu Yoo, Min Kyu Kim, Hong-Tea Kim 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 14 233-235 (3 pages)
The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and... -
GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
Hun-Su Seo, Kyoung Hee Kim, Dae-Yong Kim, Bong-Kyun Park, Nam-Shik Shin, Jae-Hoon Kim, Heejeong Youn 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 2 241-248 (8 pages)
We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/ mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal... -
Dynamic analysis of Ca level during bovine oocytes maturation and early embryonic development
Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 5 133-142 (10 pages)
Mammalian oocyte maturation and early embryo development processes are Ca-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca was distributed in the cortical ooplasm and throughout the oocytes from... -
pH-dependent modulation of intracellular free magnesium ions with ion- selective electrodes in papillary muscle of guinea pig
Shang-Jin Kim, In-Gook Cho, Hyung-Sub Kang, Jin-Shang Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 5 31-36 (6 pages)
A change in pH can alter the intracellular concentration of electrolytes such as intracellular Ca2+ and Na+ ([Na+]i) that are important for the cardiac function.For the determination of the role of pH in the cardiac magnesium homeostasis, the intracellular Mg 2+ concentration ([Mg 2+]i), membrane potential and contraction in the papillary muscle of guinea pigs using ion-selective electrodes changing extracellular pH ([pH]o)or intracellular pH ([pH ]i )were measured in this study. A high... -
Human embryonic stem cells and therapeutic cloning
Woo Suk Hwang, Byeong Chun Lee, Chang Kyu Lee, Sung Keun Kang 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 1 87-96 (10 pages)
The remarkable potential of embryonic stem (ES) cells is their ability to develop into many different cell types. ES cells make it possible to treat patients by transplanting specialized healthy cells derived from them to repair damaged and diseased cells or tissues, known as “stem cell therapy”. However, the issue of immunocompatibility is one of considerable significance in ES cell transplantation. One approach to overcome transplant rejection of human ES (hES) cells is to derive hES cells... -
Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos
Gook-jun Ahn, Byeong-chun Lee, Woo-suk Hwang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 11 131-137 (7 pages)
For parthenogenetic activation as a model system of nuclear transfer, microinjection and electroporation as activation treatments in bovine metaphase II oocytes were administered to each of three groups as follows: control group (treatments with Ca2+, Mg2+ -free PBS+100 micro M EGTA), IP3 group (control+25 micro M IP3) and IP3+ ryanodine group (control+25 micro M IP3+10 mM ryanodine). In experiments using microinjection, no significant differences were observed between any of the developmental... -
Extracellular acidity enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis via DR5 in gastric cancer cells
Ran Hong, Song Iy Han 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.5 5 513-523 (11 pages)
The tumor microenvironment greatly influences cancer cell characteristics, and acidic extracellular pH has been implicated as an essential factor in tumor malignancy and the induction of drug resistance. Here, we examined the characteristics of gastric carcinoma (GC) cells under conditions of extracellular acidity and attempted to identify a means of enhancing treatment efficacy. Acidic conditions caused several changes in GC cells adversely affecting chemotherapeutic treatment. Extracellular... -
Calcium permeability of transient receptor potential canonical (TRPC) 4 channels measured by TRPC4-GCaMP6s
JuyeonKo, JongyunMyeong, DongkiYang, InsukSo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.1 16 133-140 (8 pages)
Conflicting evidence has been obtained regarding whether transient receptor potential cation channels (TRPC) are store-operated channels (SOCs) or receptor-operated channels (ROCs). Moreover, the Ca/Na permeability ratio differs depending on whether the current-voltage (I-V) curve has a doubly rectifying shape or inward rectifying shape. To investigate the calcium permeability of TRPC4 channels, we attached GCaMP6s to TRPC4 and simultaneously measured the current and calcium signals. A TRPC4... -
Mitochondrial calcium uniporter inhibition attenuates mouse bone marrow-derived mast cell degranulation induced by beta-1,3-glucan
DangVanCuong, HyoungKyuKim, JubertMarquez, NariKim, KyungSooKo, ByoungDooRhee, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.2 10 213-220 (8 pages)
focused on the role of the mitochondrial calcium uniporter (MCU) in BG-induced degranulation. Black mouse (C57) bone marrow-derived mast cells were stimulated with 0.5 μg/ml BG, 100 μg/ml peptidoglycan (PGN), or 10 μM A23187 (calcium ionophore), and dynamic changes in cytosolic and mitochondrial calcium and membrane potential were monitored. BG-induced mast cell degranulation occurred in a time-dependent manner, and was significantly reduced under calcium-free conditions. Ruthenium red, a...
