As part of an extensive program on the microbiological oxidation of hydrocarbons, reaction conditions and nutrients consumption of candida lipolytica grown on alkane as carbon source were studied. For optimum growth of yeast, the conditions of pH 5, temperature $30^{circ}C$, carbon number $C_{16}$& $C_{18}$aeration 25.6 1/l/hr, agitation 3000 r.p.m., hydrocarbon concentration 10% were obtained. Carbon and nitrogen balance related to these conditions were also investigated. In the course of this investigation, some significant effects of pH and carbon number of hydrocarbon variation were observed.