The cultural conditions of the osmophilic yeasts for higher concentration of NaCl selected in the previous report were examined and the results obtained were as follows. 1) The strain $T_3;and;T_8$ were grown exceedingly well on the media containing 15 percent of NaCl and $T_5;and;T_9,;T_{10};and;T_{11}$ on the media containing 5 percent of NaCl. 2) The optimum temperature for growth of the strain $T_3;and;T_5$ was $30^{circ}C,;T_8;T_{10}$ and $T_{11};was;25^{circ}C;and;T_9;was;35^{circ}C.$ 3) Their lethal temperature was $60^{circ}C$ (treatment for 10 minutes). 4) The optimum pH for growth of the strain $T_3;and;T_8$ was pH 4.0, $T_5$ was pH 6.0 and $T_9;T_9;and;T_{11}$ was pH 5.0, respectively.