Resistance to Black shank of various varieties were investigated in field and incubator. The results were summarized as follows; 1) In occurrence of this disease, yellow tobacco varieties were ranged from 4 days to 12 days after inoculated. 2) Korean native varieties were ranged from 6 days to 20 days. 3) Orient tobacco takes from 8 days to 17 days in occurrence of this one. 4) Japanese varieties ranged from 4 days to 3 days in occurrence of this disease. 5) Dark air-curing tobacco was ranged from 5 days to 14 days. 6) Chinese variety was ranged from 6 days to 11 days. Each varieties of resistant to Black shank was as follows; 1) Yellow tobacco varieties; Cash, Oxford-1, Oxford-3, N.C-75, N.C-95, Golden-cure Delcrest. 2) Native varieties; Sinzongtchio, Muktchio Sosa-3 Mokgitchio. 3) Japanese varieties; Hatano Outaruma Maruha. 4) Chinese variety; Gkzuoe, Yung-zung, Tongzuoe. 5) Orient tobacco; Samsun, Baffra, Xanthix. 6) Dark air-curing varieties; B-Hw, Connecticut-Broad leaf, Connecticut-Havana, Tennessee-Black.