1. Eight Varieties of Nicotiana tabacum, "Yellow special A", "Gold leaf", "Oxford, " "Awha", "Yungzung", "Samsun", "Maruha" and "Hatano" were compared on the photoperiodic responses. 2. Experiments were conducted both under high temp. condition(green-house, average temp $25^{circ}C$-$35^{circ}C$) and low temp. condition (out-door; average temp $10^{circ}C$-$25^{circ}C$) in four plots of 8.10.12 and 14 hours in day length. 3. The results obtained are summarized as follows; i) In the short day condition, the flowering was accelerated in the low temp. than high. On the contrary, in the high temp, it was accelerated in the long day. ii) Generally speaking, the flowering was responsed more in low temp. and short day length than high temp.-long day. iii) But the above trend was found great difference among varieties..-long day. iii) But the above trend was found great difference among varieties.