저자들은 실험암세포인 Walker 256 carcinosarcoma로 부터 핵을 분리한 후 Johns 및 Phillips and Johns 방법에 의하여 histone분획 즉 H1, H2a, H2b, H3 및 H4 등 5개의 주분획을 분리하였다. 각 histone 분획들은 15% polyacrylamide disc gel 전기영동에 의하여 순도 및 mobility를 검토하였다. 또 각 분획의 아미노산을 정량하여 아미노산 및 methyl화된 아미노산을 분리 확인하였으며, 동물의 정상조직의 그것과 비교 검토하여 다음과 같은 결과를 얻었다. 1. 총 histone의 수율은 Walker 256 carcinosarcoma조직 1g당 3.75mg이다. 2. DNA의 함량은 조직 1g당 3.35mg으로 DNA:histone의 비는 1 : 1.12이다. 3. 실험암세포 조직으로 부터 5개의 histone분획 즉 H1, H2a, H2b, H3 및 H4 등을 분리하였으며, 이들의 상대적 백분율은 각각 20.0%, 9.33%. 26.67%. 33.33% 및 10.67%이다. 4. 전기영동상의 이동도는 다른 조직의 것과 별 차이가 없었으며, 아미노산 조성에는 약간의 차이가 있었다. 5. 동물의 정상조직에서는 아직까지 보고되지 않은 3-methylhistidine을 H1 분획에서 분리 확인하였다. 또 $varepsilon$-N-monomethyllysine을 H2a,H2b 및 H4분획에서 $varepsilon$-N-dimethyl-lysine은 H2b에서 각각 분리 확인하였다.
In attempt to investigate histone fractions of tumor cells, nuclei was isolated from Walker 256 carcinosarcoma by the procedure of Pogo et al., Histone fractions H1, H2a, H2b, H3 and H4 were prepared from isolated nuclei by the procedure of Johns, The five histone fractions found in most tissues were also present in the Walker 256 carcinosarcoma histones. These histone fractions were characterized by amino acid analysis and by polyacrylamide disc gel electrophoresis. The results obtained were as follows: 1. The yield of whole histone was 3. 75mg per g of Walker 256 carcinosarcoma. 2. The yield of DNA was 3.35mg per g of Walker 256 carcinosarcoma. Consequently the DNA to histone ratio was 1 : 1. 12. 3. The relative amounts of five fractions, i.e., H1, H2a, H2b, H3 and H4 were 20.00%, 9.33%, 26.67%, 33.33% and 10.67%, respectively. 4. The electrophoretic mobility of individual histone fractions gave almost similar patterns to those of corresponding fractions of calf thymus. 5. Amino acid analysis of the individual histone fractions showed that the over-all compositions were similar but not identical to those of the corresponding fraction from calf thymus. 6. We found that histone H2b fraction of Walker 256 carcinosarcoma contained detectable amounts of $epsilon$-Nemonomethyllysine and $epsilon$-N-dirnethyllysine. No evidence for the presence of methylated lysine or other side-chain derivatives was reported on this histone fraction. We also found that histone H1 fraction contained detectable amounts of 3-methylhistidine.
