1. Maize seedlings에서 c-AMP의 존재가 확인되었고, 그 양은 4일까지 점차 감소하였다. 2. 외부에서 넣어준 c-AMP에 의해 isocitrate lyase의 유도가 촉진됨을 알 수 있었고, endosperm의 c-AMP도 같은 역할을 예상할 수 있다. 3. Maize seedlings의 endosperm의 단백질과 지질은 3일째까지 급격히 분해되었고, 그 후에는 서서히 분해되었다. 4. Embryo의 단백질양은 3일째까지 감소하다가 그 후에는 증가되는 경향을 보였다. 5. Isocitrate lyase와 lipase활성은 3일째에 최대치에 도달하였고 그 후에는 감소하는 경향을 보였다. 6. Lipase활성은 lipid granules의 시간에 따른 변화와 일치하였다. 7. Polyamine합성효소의 활성은 발아후 5일에 최대치를 보였고, 효소 활성은 fresh weight와 단백질 양의 증가를 유도한 것 같다.
1) The presence of c-AMP was observed from germination to seedling and the amount of it was continuously decreased until 4th day. 2) Exogenous c-AMP was found to promote the induction of isocitrate lyase suggesting that endogenous could promote the enzyme. 3) The amounts of protein and lipid in endosperm were rapidly decreaced for the first 3 days and then slowly decreased. 4) The amount of protein in embryo was rapidly decreased for the first 3 days and thereafter increased. 5) Activities of isocitrate lyase and lipase increased and reached maximum in 3 days and then decreased. 6) Lipase activity was paralleled with chronoligical changes of lipid granules. 7) The enzyme activity related to polyamine biosynthesis reached maximum in 5th day after germination and its activity seemed to result in increase in fresh weight and protein amount.
