High pressure liquid chromatography를 이용하여 돼지의 adrenal medulla로 부터 leucine 및 methionine enkephalin을 분리 한 후 아미노산 조성 및 아미노산 배열을 규명한 실험 결과를 얻었다. 1. 이들 아편성 peptide의 순수 분리에는 gel chromatography, CM-cellulose chromatography, thin layer chromatography, HPLC를 이용하였다. 2. peptide인 leucin enkephalin과 아미노산 조성의 몰 비율은 Tyr : 1.00, Gly : 1.96, Phe : 1.03, Leu: 1.03, Leu: 1.00. Methionine enkephaline은 Tyr : 1.00, Gly : 1.98, Phe : 1.02, Met: 1.01 이었다. 3. pentapeptide인 leucine 및 methionine enkephalin을 medulla로부터 추출 분리하였으며, 아미노산 배열은 H-Tyr-Gly-Gly-Phe-Leu-OH; leucine enkephalin, H-Tyr-Gly-Gly-Phe-Leu-OH였다.
Leucine and methionine enkephalin was isolated from porcine adernal medulla by use of high pressure liquid chromatography (HPLC), and the amino acid composition and sequence of isolated peptides were elucidated. 1. We empolyed gel chromatography, CM-Cellulose chromatography, thin layer chromatography, amino acid analysis and HPLC for the purificatition of opioid peptides. 2. The molar fraction ratio of amino acids of pentapeptides, leucine enkephalin, is shown to be Tyr: 1.00, Gly: 1.96, Phe: 1.03, leu: 1.00, and that of methionine enkephalin is shown to be Tyr: 1.00, Gly: 1.98, Phe: 1.02, Met: 1.01. 3. Leucine and methionine enkephalin has been isolated from acid exttracts of porcine adrenal medulla and found to have the amino acids sequence H-Tyr-Gly-Phe-Leu-OH and H-Tyr-Gly-Gly-Phe-Met-OH.
