쥐 뇌의 Peroxidase 활성도를 o-dianisidine을 이용 분광학적 방법으로 측정했다. 유사 peroxidase를 제거하기 위해 기본적인 효소측정의 여건을 살폈으며 이에 따라 세포내 및 뇌의 여섯 부위에서 peroxidase 활성도를 검토했다. 대부분의 활성도는 particulated fraction에서 발견됐으며 microsome의 고유 활성도는 mitochondria에 비해 약 두배였다. Microsome 활성도의 뇌 부위별 분포는 상당히 변화가 있어 medulla oblongata에서 가장 높았고 striatum에서는 가장 낮은 활성도를 보여 주었다. 뇌 peroxidase의 가능한 역활에 대하여 간단히 평가했다.
The peroxidase activity of rat brain was determined by spectrophotometrically using o-dianisidine as substrate. Basic assay conditions were optimized in order to eliminate the pseudoperoxidase activity. The peroxidase activities were then examined in subcellular fractions as well as in six different regions of the brain. It was found that a major part of the activity was associated with particulated fractions. The specific activity of the microsomal enzyme was roughly twice as high as that of the mitochondrial. The regional distribution of the microsomal activity was quite variable. The highest activity was found in the medulla oblongata and lowest in the striatum. The possible role of the brain peroxidase was birefly assessed.
