쥐의 적혈구에 대한 인삼 사포닌의 용혈 활성과 그 침투 여부를 조사하고 미숙 적혈구에서의 단백질 합성에 미치는 인삼 사포닌의 영향을 관찰하여 다음과 같은 결과를 얻었다. 1. 적혈구를 여러 가지 농도의 인삼 사포닌 분획을 포함한 등장액에 방치하였을 때 사포닌의 농도가 $1{ imes}10^{-3}%$ 이하일 때는 용혈 활성이 전혀 관측되지 않았으며, $10^{-2}%$ 이상에서는 현저한 용혈 활성을 나타내었고, $3{ imes}10^{-3}%$ 때는 50%의 용혈을 나타내었다. 2. 적혈구를 $^{14}C$으로 표지된 방사성 인삼 사포닌($0.014;{mu}Ci$)을 함유한 $10^{-4}%$ 사포닌 분획 용액에 10분간 방치하면 가해준 사포닌의 약 19%가 적혈구내로 침투되고 그 대부분은 세포질에 존재함이 관측되었다. 3. 적혈구를 $10^{-4}%$ 인삼 사포닌 분획 용액에 10분간 방치한 후 분리한 시토졸의 Glucose 6-phosphate dehydrogenase의 활성이 크게(20%) 증가된 것으로 보아도 침투된 인삼 사포닌이 원형질 분획에 존재함을 알 수 있었다. 4. 사혈성 빈혈을 일으킨 쥐에서 얻은 미숙 적혈구에서의 $^{14}C$-leucine의 단백질로의 편입도 $10^{-4}%$ 사포닌 분획 용액에서 촉진(20%)되었음이 관찰되었다.
It was attempted to observe the uptake of ginseng saponin by red blood cells in this study. The effect of the saponin on protein biosynthesis using immature red blood cells of rats was also investigated and the fallowing results were obtained. 1. Red blood cell suspension in isotonjc buffer was incubated in ginseng saponin solution of various concentrations and the 50% hemolysis was found to be $3{ imes}10^{-3}%$. 2. When the red blood cell suspension was incubated with $10^{-4}%$ ginseng saponin containing radioactive saponin ($0.014;{mu}Ci$) about 19% of the total saponin was taken up by the cells and most of them were in cytosol fraction. 3. When glucose 6-phosphate dehydrogenase was assayed in the presence of various amounts of ginseng saponin, it was found that the activity was stimulated in $10^{-5}%$ saponin solution. 4. Glucose 6-phosphate dehydrogenase of the red cells which were incubated in $10^{-4}%$ saponin solution was found to be stimulated (20%). 5. When immature red blood cells were incubated in reaction mixture containing $10^{-4}%$ saponin, it was found that the protein biosynthesis was stimulated about 124% of the control.
