DMBA의 대사과정에 관련하는 효소와 체내 방어기전에 인삼 ether추출물과 selenium이 어떤 영향을 미쳐 돌연변이가 억제되는지 알아보기 위해 인삼 (2 mg/kg)과 selenium ($5;{mu}mol/kg$)을 10일간 흰 쥐에 투여하였다. 이때, GST와 GSH-px활성은 각각 12.3%, 186% 증가하였으며 DMBA-induced lipoperoxide 형성도 억제되었다. Ames 방법으로 DMBA ($205;{mu}g/plate$)의 돌연변이원성에 미치는 영향을 조사한 결과, 인삼투여군은 17%, selenium투여군은 22%, 동시투여군은 41%까지 억제하였으며 시험관 내에서 인삼농도가 0.5~2 mg/plate일때 14~38%, selenium 농도가 $1{sim}4;{mu}mol/plate$일 때 32~66% 억제됨을 알 수 있었다.
Glutathione S-transferase and glutathione peroxidase are a group of multifunctional enzymes that involved in the metabolic conversion of DMBA. When ginseng ether extracts (2 mg/kg, i.p., 10 days) and selenium ($5;{mu}mol/kg$, i.p., 10 days) to rats, GST and GSH-px activities showed by 12.3%, 186% as compared with the normal group respectively. Also, this materials inhibited on the formation of DMBA-induced lipoperoxide. Antimutagenic effects of ginseng ether extracts, selenium, and ginseng+selenium in the DMBA ($20;{mu}g/plate$) inducing mutagenicity using Salmonella typhimurium strain TA 98 by the Ames method exhibited by 17%, 22%, and 41%, respectively. Direct additions of ginseng ether extracts (0.5~2 mg/plate) and selenium ($1{sim}4;{mu}mol/plate$) with S-9 mixture to the mutagenicity assay system showed 14~38%, 32~66% inhibition of DMBA mutagenicity, respectively.
