Sprague Dawley 쥐 뇌의 opiate receptor의 날록손 결합 양상을 Scatchard plot 하였을때 high affinity binding과 low binding을 나타내었다. High affinity binding sites의 Bmax는 8.79 fmol/mg protein이었고 low affinity binding sites의 dissociation 상수 (Kd)는 9.09 nM 이었다. Brain membrane을 phospholipase $A_2$와 반응시켰을때 Bmax는 7.13 fmol/mg으로 Kd는 0.32 nM로 감소하였다. 1, 6, 12주 된 쥐 뇌막에서의 phosphatidylserine 양, lipid peroxide 형성과 날록손 결합 양상에 대한 변화를 관찰하였다.
Apparant two binding sites, high and low affinity, were shown when Scatchard plotted after the rat brain membrane was analyzed for $^3H$-naloxone binding characteristics with opiate receptors. The maximum number of high binding sites (Bmax) was 8.79 fmol/mg brain membrane protein while the low affinity binding sites displayed the apparent dissociation equilibrium constant (Kd) of 9.09 nM. When the brain membrane was treated with phosphalipase A, Bmax has reduced to 7.13 fmol/mg and Kd to 0.32 nM. The changes in binding parameters with 1, 6, 12 weeks old rat brain were observed under various experimental conditions.
