흰쥐 성장과정 중에 cytochrome P-450의 달라지는 발현(expression)과 다양성을 RIA와 Western blotting에 의하여 관찰하였다. Non-specific MAbs (NBS 1-48-5와 HYEl-9), cytochrome P-450 2c/RLM5에 대한 MAbs (1-68-11)을 비교하였더니 대부분의 숫컷 어린쥐와 성숙된 쥐 cytochrome P-450 2c/RLM5와 일치 하는 것을 확인하였다. 그리고 양적인 순서는 ethanol-, isosafrole- 및 phenobarbital-로 유도된 cytochrome순이었다. 정제된 cytochrome P-450 2c/RLM5에 대한 MAbs와 숫컷 성숙된 쥐 간조직 마이크로좀에서 immunopurify한 cytochrome P-450은 같은 pattern을 나타내었다. 이런 결과는 정제된 cytochrome P-450이 homologus 하거나 화학적으로 정제된 cytochrome 2c/RLM5에 공통된 epitope를 갖는다는 것을 지적하는 것이다. 또한 cytochrome P-450 2c/RLM5에 대한 MAbs같이 Western blotting도 비슷한 결과를 얻었다.
We observed the multiplicity as well as differential expression of cytochrome P-450 during the development of rats detected by RIA and Western blotting. Compared to non-specific MAbs (NBS 1-48-5 and HYEL-9), MAbs to cytochromes P-450 2c/ RLM5(1-68-11) recognized the Corresponding cytochrome P-450 2c/RLM5 the most in young and adult male and the following cytochromes P-450 in their quantity detected by respective MAbs were ethanol-, isosafrole-and phenobarbital-inducible cytochrome P-450. When MAbs to cytochrome P-450 2c/RLM5 were purified and cytochrome P-450 was immunopurified from the liver microsomes of adult male rats with the purified MAbs, the concentration dependent MAb-bindings to both chemically and immunopurified cytochrome P-450 showed similar patterns. The highly expressed form of cytochrome P-450, P-450 2c/RLM5 was immunopurified from liver microsomes of adult male rats with purified MAbs and it was confimed that both chemically purified cytochrome P-450 were homologous or at least contained common epitope to cytochrome P-450 2c/RLM5. Similar results were obtained in Western blotting with MAbs to cytochrome P-450 2c/RLM5.
