Azospirillum lipoferum SK16에서 tryptophan으로부터 indole-3-acetic acid (IAA)의 생합성 경로를 규명하기 위하여 tryptophan을 첨가하여 배양한 배양액 및 초음파 파쇄한 세포추출물에서 IAA 합성 중간대사물들을 TLC 및 HPLC 로 분석한 결과 tryptophan$ ightarrow$indole-3-pyruvate$ ightarrow$indole-3-acetaldehyde${ ightarrow}$IAA의 주 생합성 경로가 확인됨과 동시에 tryptophan에서 tryptamine을 거치는 부수적인 합성경로도 있는 것으로 추정되었다.
The synthetic pathway of indole-3-acetic acid (IAA) from tryptophan in Azospirilium lipoferum SK16 was examined by analysis of IAA intermediates with thin layer chromatography (TLC) and high performance liquid chromatography(HPLC). Analysis of the IAA intermediates from A. lipoferum SK16 culture broth and cell extract by the TLC and HPLC have indicated that the major synthetic pathway from tryptophan to IAA is via tryptophan$ ightarrow$indole-3-pyruvate$ ightarrow$indole-3-acetaldehyde$ ightarrow$IAA. For the confirmation of the pathway, tryptophan aminotransferase, the enzyme catalyzing the first reaction from tryptophan to IAA, was examined in cell free extract and the result of the enzymatic analysis Was well coincided with the data of IAA intermediate analysis in the culture broth. Minor pathway of IAA synthesis via tryptamine was also speculated in A. lipoferum SK16.
