Recently, some reports of experimental research on the distribution of fatigue crack propagation rate have been published, and the reliability evaluation using the results of research for the mechanical structure has been executed. Since the thicknesses of specimens used in the published reports are limited to the thin ones, the applicability of the results into the mechanical structure with another thickness seems to be doubtful. That is, not only the quantitative evaluation, but also qualitative evaluation of the effect of specimen thickness has not been executed. In this study, an experimental investigation has been done by using the new type automated multi-stage fatigue testing machine which was developed by the author. The influence of specimen thickness for the distribution of fatigue crack propagation rate with the results is discussed.