Glycine max를 24시간 물에 담그면서 여러 형태의 polyamine을 관찰한 결과 결합형의 polyamine들이 conjugated form이나 자유형에 비해 많이 존재하였다. 주 저장단백질인 glycinin을 정제한 후 이들을 가수분해하여 polyamine을 분석한 결과 putrescine, spermidine, spermine을 각각 glycinin 1g당 $14.0{sim}16.2{mu}mol$, $18.8{sim}22.4{mu}mol$, $4.4{sim}7.3{mu}mol$ 함유하고 있었다. Putrescine에 대한 항체로 immunocytochemical localization을 수행한 후 전자현미경으로 관찰하였더니 대부분의 면역학적인 반응성은 단백질체에서 나타났다.
Quantitative analysis of different forms of polyamines was accomplished in Glycine max, seed soaked in water for 24 h. The content of bound polyamine was turned out to be higher than those of free and conjugated polyamines. Acid hydrolysates of glycinins purified from soybean cotyledons showed that 1g of the glycinin contained$14.2{sim}16.0{mu}mol$ of put, $18.2{sim}22.3{mu}mol$ of spermidine, and $4.4{sim}7.3{mu}mol$ of spermine, respectively. Through immunocytochemical localization of putrescine it was found in vacuolar protein bodies, which contain glycinin.
