To establish effectively for isolation and cultivation and cultivation of scuticociliata, we tried to culture scuticociliata using culture medium(P2Y1-DW medium). The number of scuticociliata reached $1.50{ imes}10^3$ cells/ml at the temperature of $25^{circ}C$ in 3 days, but $1.03{ imes}10^2$ cells/ml at $15^{circ}C$. In P1Y1-S medium growth reached $2.6{ imes}10^4$cells/ml in 3 days, but the number in DW medium was the lowest with $2.0{ imes}10^2$cells/ml. In all these media, the number decreased after 5 days. As the method of the parasite`s division, after attaching the anterior part of the body, its shape gradualy changed to a western pear-like appearance. The parasite completely divided into two cells, then freely swam and became an adult cell, size of $40{mu}m$ in size within about 3 hours from attaching.