Ti alloys, with the advantageous tensile strength/density ratio and the chemical stability, have been used widely in the aerospace and chemical engineering industries and their usages are still expanding in various industrial areas. In the automotive industry, because of their superior merits of weight reduction and fuel saving, Ti alloys are expected to be used as various part materials including connecting rods, engine valves, springs and retainers, which are all subjected to the fatigue loads. In this study, using Ti-3A1-2.5V, the effects of temperature and microstructure change on fatigue crack propagation has been investigated. Five different microstructures were tested at the temperatures of room temperature, 20$0^{circ}C$, 30$0^{circ}C$ and 40$0^{circ}C$ under the same frequency 20Hz. Some of the conclusions obtained are as follows: (1)Microstructurally, the morphology of less $alpha$-phase and finer lamellar structure of $alpha$ and $eta$-Ti showed better registance to the fatigue crack propagation. (2)Fatigue crack growth rate increased with test temperature.