Two methods were used to detrermine the net photosynthetic rate(NPR) in the plug stand using a wind tunnel for plug seedlings Production. One is called as the integration method in which NPR calculated by the use of air current speed and $CO^2$ concentration measured at any heights above the medium surface in a wind tunnel were summed. It was assumed that the air flow at any layer did not mix with the lower or upper air layer. The other is called as the diffusion method in which eddy diffusivities above the plug stand were used to determine the NPR in the plug stand. In this method, $CO^2$ above or inside the plug stand was assumed to be absorbed vertically. NPR determined by the diffusion method was 28~45% of the NPR calculated by the integration method. Considering the magnitude of NPR and the effects of the air current speed on NPR, the integration method would be adequate for the calculation of NPR in the plug stand. Maximum NPR determined using the integration method appeared at the air current speed of 0.7m $s^{-1}$. It was ascribed to the decreased diffusion resistances of $CO^2$ with the increasing air current speed. NPR at the rear region in plug stand was 20~34% lower than that at the front region. NPR sharply decreased with the increase of an elapsed time after the beginning of photoperiod. Therefore $CO^2$ enrichment would be effective to force the growth of plug seedlings in a semi-closed ecological system under artificial lighting.