This study was conducted to develope a machine vision system for a robotic transplanter for bedding plants. Specific objectives of this study were 1) to get coordinates of the healthy seedlings in high-density plug tray, and 2) to get the angle of the leaves of the healthy seedlings to avoid the damage to seedlings by gripper. Results of this study were summarized as follows. (1) The machine vision system of a robotic transplanter was developed. (2) Success rates of detecting empty cell and bad seedlings for 72-cell and 128-cell plug-trays were 98.8% and 94, 9% respectively. (3) Success rates of calculating the angle of leaves fer 72-cell and 128-cell plug-trays were 93.5% and 91.0% respectively.