Yeung Nam University Medical Center, Department Of Central medical Supply Background: The adverse effect of intravenous therapy combined with various complications. Because sterilization technique and appropriate nursing care can prevent various complications, it is important to use appropriate sponge in intravenous therapy. The purpose of study was to identy sterilization effect and detection of bacteria and to provide basic data for use of appropriate disinfectants. Methods: From May 15 1995 to Aug. 3. 1995, disinfectants that were used in Yeung Nam University Medical Center were tested by bacteria culture. To test sterilization effect of disinfectants of intravenous injection sites after disinfection, 10 subjects were used and were tested by bacteria culture for the study. Results: 1) By sterilization effect of disinfectants, bacteria were increased from 103 to 10 from 48 hours in both 2% Zephanon and 2% zephanon that was sterilized by steam, from 10 to 10 from 72 hours and 10 from 48 hours in 70% lsoprophyl alchol. Also, bacterias were detected in 70% lsoprophyl alchol on 48 hours. 2) By stenlization effect of sponge that were used in nursing unit, bacterias were detected in 2% Zephanon on 2 hours, 70% lsoprophyl alchol on 2 hours, 70% lsoprophyl alchol on 8 hours and 70% lsoprophyl alchol on 48 hours. 3) By sterilization effect and detection of bacteria of intravenous site after disinfection, bacterias were detected in 10 of 10 control groups, 8 of 10 sites that were disinfected by steam sterilized 2% zephanon sponge, 6 of 10 sites that were disinfected by 70% lsoprophyl alchol and 4 of 10 sites that were disinfected by 10% Batadine. Conclusions : it is conclued that 70% lsoprophyl alchol is appropriate for intravenous therapy and 10% Betadine is appropriate in ward that were polluted the air and in immunodeficient patients.