배추흰나비의 5령 유충의 혈림프로부터 2개의 Iysozyme을 분리하여 아미노산 서열을 분석한 결국 두 Iysozyme의 아미노산 조성의 차이에도 불구하고 20번 아미노산잔기까지 동일하였다. 이러한 Iysozyme의 체내 분포와 발생 단계별 농도 변화 및 다른 종과의 상호관계를 면역학적 방법으로 분석하였다.
We have previously isolated two Iysozymes, Iysozymes I(LI) and II(LII) from the immune haemolymph of the fifth instar larvae of cabbage butterfly, Afogeia rapae [Yoe, S.M., Bang,I.S., Kang, C.S. and Kim, H.J. (1996) Mot. Cells 6,609-614]. Lysozymes with hinnavins are the main types of the antibacterial proteins of cabbage butterfly. The N-terminal sequences of the two Iysozymes were determined by the automated Edman degradation and their N-terminal sequences up to the 20th residue were completely identical, although LI and LII show considerable differences in amino acid compositions. Organ distribution, developmental change and immunological relationship of these enzymes were investigated through immunoelectrophoresis test and Western blot analysis.
