목적: Manganese dipyridoxyl diphosphate(Mangafodipir trisodium, Teslascan, Nycomed, 이하 Mn-DPDP)가 간의 자기공명영상(이하 MRI)과 양자 자기공명분광(이하 MRS)에 미치는 대상과 방법: 시험관연구로서, 4.7 테스라자기공명영상기기를 이용하여 다양한 농도의 지질용액에 Mn-DPDP를 가하기전과후에 MRS 스펙트럼을 얻어 1.3 ppm 지질피크의 변화를 보았다. 생체연구로서, 가토간조직의 망간함량변화를 알아보기 위해 Mn-DPDP(10 $mu$mol, 1ml/kg) 를 가토에 주사하기 전과 주사 후 10분, 20분, 30분, 1시간, 2시간, 4시간, 24시간에 간조직을 원자흡광분광분석기로 분석하였다. 같은 시간에 T1-강조영상을 얻어 간의 신호강도를 구하고 상대적조영증강정도를 계산하였으며, MRS스펙트럼을 얻어 각 대사산물들의 피크면적을 구하고 이를 지질피크면적으로 나눈 상대비를 계산하여 간조직의 망간함량변화와 비교하였다. 결과: 시험관내 연구에서 Mn-DPDP는 1.3 ppm의 지질피크를 억제하는 효과를 보였다. 간조 직의 망간함량은 Mn-DPDP 주사 후 차츰 증가하여 20분에 최고에 도달하고 점차 감소하여 24시간에 정상수준에 도달했다. Mn-DPDP 주사 후 T1-강조영상에서 간조직의 신호강도변 화는 간조직의 망간함량변화와 유사한 형태를 보였다. MRS에서 Mn-DPDP 주사 후 1.3 ppm의 지질피크면적은 간조직의 망간함량과 역으로 변했다. 결론: Mn-DPDP는 MRI의 T1-강조영상에서 간의 조영증강효과를 보이고, MRS에서는 1.3 ppm의 지질피크를 감소시키며 이는 간조직의 망간함량변화와 잘 일치하였다.
Purpose: To determine the influence of manganese dipyridoxyl diphosphate (Mn-DPDP) on MRI and proton MRS. Materials and Methods: In an in-vitro study designed to determine changes in the lipid peak at 1.3 ppm, 4.7T MR equipment was used to obtain proton MR spectrographic images of a lipid solution of varying concentration, with and without Mn-DPDP. Before; at 10, 20, and 30 minutes; and at 1, 2, 4, and 24 hours after the IV injection of Mn-DPDP (10${mu}$mol, 1ml/kg), the concentration of Mn in liver tissue was measured by atomic absorption spectrometry. At the same intervals, T1-weighted MR images were obtained, the signal intensity of the liver was thus determined, and the relative enhancement ratio was calculated. MRS of rabbit liver was performed serially at the same intervals, and the peak areas of metabolites, as well as their peak areas relative to lipids, were calculated. The findings were correlated with tissue Mn concentration. Results: At 1.3 ppm with Mn-DPDP, MRS showed that the peak area of the lipid had decreased. Tissue Mn concentration increased just after Mn-DPDP injection and peaked after 20 minutes, decreasing to a level within the normal range after 24 hours. Serial changes in the signal intensity of the liver, as seen at MRI, showed similar pattern to that of Mn concentration. There was reverse correlation between serial change in the peak area of lipids at 1.3 ppm and Mn concentration after Mn-DPDP injection. Conclusion: At T1-weighted MR imaging, the injection of Mn-DPDP led to the enhancement of liver tissue, and at MRS, the lipid peak at 1.3 ppm decreased. There was close correlation between these effects and tissue Mn concentration.
