In this study, the spray cooling heat transfer and working characteristics of the screen wick heat pipe with ultrasonic spray cooling system in condenser were experimentally investigated. The heat pipe was made of copper tube 300 mm long with inner diameter of 11.1 mm. The evaporator and condenser lengths of heat pipe were 40, 200 mm and the wick structure consists of two layer of 100 mesh copper screen. The experimental results show that the ultrasonic spray cooling increases the heat transfer rate on the condenser surface, and the total thermal resistance of heat pipe system decreases remarkably. A comparison is made for the two working fluids, water and ethanol. The surface temperature of the ethanol tube in evaporator section becomes higher than that of the water tube. Thus, the experimental result shows that water is more useful than ethanol as the working fluid because of increasing the operational limit within this experimental conditions.