Near infrared reflectance spectroscopy (NIRS) is a rapid and accurate analytical method for determining the composition of agricultural products and feeds. This study was conducted to determine tocopherol and tocotrienol contents in rice bran by using NIRS system. Total 80 rice bran samples previously analyzed by HPLC were scanned by NIRS and over 60 samples were selected for calibration and validation equation. A calibration equation calculated by MPLS(modified partial least squares) regression technique was developed and coefficient of determination for tocopheyol and tocotyienol content were 0.975 and 0.984, respectively, in calibration sets. Each calibration equation was fitted to validation set that was performed with the remaining samples not included is the calibration set, which showed high positive correlation both in tocopherol and tocotrienol content file. This results demonstrate that the developed NIRS equation can be practically used as a rapid screening method for quantification of tocopherol and tocotrienol contents in rice bran.